CAPTO CORE ISO
Currently the Company employs 37 people with an R&D laboratory facility in Leiden and a state-of-the-art ISO 9001 certified manufacturing plant in Naarden, the Netherlands. Capto brings the leadership, mindset and skills garnered from a diverse M&A advisory practice to every business strategy, acquisition target, technology innovation and sourcing. BAC BV was established in 1995 as a Unilever subsidiary and is collaborating with the leading life sciences vendors. Here's how we do it: We focus on dramatically improving the operation, economics and effectiveness of client companies using our vetted proprietary SYNAPTIC methodology. They enable a reduced time to market, increased purity and yield and an overall lower cost of goods for biopharmaceutical drug development. Based on BAC's proprietary single-chain antibody technology called CaptureSelect, the products are suitable for the discovery, preclinical and clinical manufacturing of therapeutic proteins, including antibodies and antibody fragments. Capto Core 700 is also available as a bulk medium in a range of pack sizes from lab scale to production-scale.īAC discovers, develops and manufactures separation and affinity purification products for use across the value chain of biopharmaceutical R&D and manufacturing. The prepacked HiTrap™ and HiScreen™ formats simplify process development and scale-up. With Capto Core 700, flowthrough chromatography and a wide window of operation enable straightforward optimization. Smaller impurities bind to the internalized ligands, which are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The inactive outer layer excludes large molecules (Mr 700 000 and above) from entering the beads which can subsequently be collected in the column flowthrough. The Capto Core 700 medium employs core bead technology, with a ligand-activated core and inactive outer layer. Optimization and scale-up are both straightforward. Productivity is improved with up to 100-fold greater sample loadings and significantly higher flow rates. Capto Core 700 offers a range of performance advantages over a typical gel filtration step. GE Healthcare's Capto Core 700 is a multimodal chromatography medium (resin) designed for intermediate purification and polishing of viruses and other large biomolecules. This information could be used to predict and optimize the purification of large biomolecules and bioparticle in route to the establishment of more effective downstream processes.High productivity alternative to group separation for intermediate purification and polishing, offering higher sample loads and flow rates than gel filtration This study provides qualitative and quantitative information about Capto™ Core 700 resin. The DBC 10% at 2 min residence time are 24 and 2 mg/mL for BSA and Tg, respectively. Effective pore diffusivities values in the core are 1.6 × 10 −7 and 0.16 × 10 −7 cm 2/s for BSA and Tg, respectively. For both proteins, the effective pore diffusivity in the core is smaller than in the shell due to additional hindrance by bound protein in the core area. The addition of 500 mM NaCl reduces the binding capacity by less than 50%, showing the ability of the resin to operate at high salt conditions. Both proteins present highly favorable binding isotherms with maximum binding capacities of 55 and 105 mg/mL of total bead volume for BSA and Tg, respectively. The resin average bead size is 90.7 μm with a range of 50–130 μm, the shell thickness is 4.18 µm with a range of 3–6 µm and a standard deviation of 0.55 µm, and the pore radius, obtained by inverse size exclusion chromatography, is 50.4 ± 1.3 nm.
The functionalized adsorbing core and the inert shell have the same fibrous structure typical of agarose-based beads. The present study aims to characterize the structure and functional properties of this resin using bovine serum albumin (BSA, Mr~65 kDa) and thyroglobulin (Tg, Mr~660 kDa) as model impurity proteins. Capto™ Core 700 is a core-shell chromatographic support with an adsorbing core contained within an inert shell layer designed to purify larger biomolecules and bioparticles in a flow-through mode.